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phusion hf buffer  (New England Biolabs)


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    New England Biolabs phusion hf buffer
    Phusion Hf Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1649 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phusion hf buffer/product/New England Biolabs
    Average 96 stars, based on 1649 article reviews
    phusion hf buffer - by Bioz Stars, 2026-06
    96/100 stars

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    (A) Genetic homology of POWV strains LB and LI9 with differences indicated by white bars. (B) LB genomes (∼11 kb) were cloned into 5 overlapping fragments and purified <t>Phusion</t> amplified fragments were used develop reverse genetics. (C) POWV-LB fragments and UTR linkers, containing an HDVr, SV40pA, and CMVd2 promoter, were CPER circularized prior to transfection. (D) Immunoperoxidase staining of recLB infected VeroE6 cells 2-4 dpi. (E) Immunoperoxidase staining of WT LB or recLB infected VeroE6 cell foci (MOI, 0.1) 4 dpi.
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    (A) Genetic homology of POWV strains LB and LI9 with differences indicated by white bars. (B) LB genomes (∼11 kb) were cloned into 5 overlapping fragments and purified Phusion amplified fragments were used develop reverse genetics. (C) POWV-LB fragments and UTR linkers, containing an HDVr, SV40pA, and CMVd2 promoter, were CPER circularized prior to transfection. (D) Immunoperoxidase staining of recLB infected VeroE6 cells 2-4 dpi. (E) Immunoperoxidase staining of WT LB or recLB infected VeroE6 cell foci (MOI, 0.1) 4 dpi.

    Journal: bioRxiv

    Article Title: Powassan Virus LB Neurovirulence and Lethality is Determined by Envelope Protein Domain III Residues

    doi: 10.64898/2026.03.26.714546

    Figure Lengend Snippet: (A) Genetic homology of POWV strains LB and LI9 with differences indicated by white bars. (B) LB genomes (∼11 kb) were cloned into 5 overlapping fragments and purified Phusion amplified fragments were used develop reverse genetics. (C) POWV-LB fragments and UTR linkers, containing an HDVr, SV40pA, and CMVd2 promoter, were CPER circularized prior to transfection. (D) Immunoperoxidase staining of recLB infected VeroE6 cells 2-4 dpi. (E) Immunoperoxidase staining of WT LB or recLB infected VeroE6 cell foci (MOI, 0.1) 4 dpi.

    Article Snippet: DNA fragments 1-5 and linker (0.09 pmol each) were CPER amplified in a 25-μL reaction containing 200 μM of dNTPs, Phusion polymerase GC reaction buffer, and 0.5 μL Phusion polymerase (New England Biolabs).

    Techniques: Clone Assay, Purification, Amplification, Transfection, Immunoperoxidase Staining, Infection